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Molecular Biology Techniques,
Edition 5 A Classroom Laboratory ManualEditors: By Sue Carson, Heather B. Miller, D. Scott Witherow and Melissa C. Srougi
Publication Date:
09 Jan 2026
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Molecular Biology Techniques: A Classroom Laboratory Manual, Fifth Edition, is an in-depth laboratory manual for upper-level undergraduates and beginning graduate students in the general field of molecular biology techniques and biochemical applications. Presented as a series of coordinated experiments that mimics either a one- or two-semester research experience, the book also provides theories and explanations behind the techniques. Topics include manipulation of recombinant DNA, protein expression and purification, analysis of mRNA levels, mammalian cell culture techniques, CRISPR-mediated gene knockdown, and more. New topics, such as Gibson assembly experiments, have also been included. For instructors, the book contains easy to follow guides and preparation lists, designed to facilitate lab setup and pre-lab questions for use as a teaching tool.
Key Features
- Presents student-tested labs proven successful in real classroom laboratories
- Covers updated lab sessions that focus on silico methods
- Includes quizzes, discussion questions and further online resources for students, as well as lab preparation lists for instructor use
- Also includes updates such as Gibson assembly experiments and lab session flow charts for easier selection
About the author
By Sue Carson, Department of Plant and Microbial Biology, North Carolina State University, Raleigh, USA; Heather B. Miller, Department of Chemistry, High Point University, High Point, NC, USA; D. Scott Witherow, Department of Chemistry and Biochemistry, The University of Tampa, Tampa, FL USA and Melissa C. Srougi, Department of Molecular Biomedical Sciences and the interdisciplinary Biotechnology Program at NC State University
1. DNA MANIPULATION Lab session 1-5
2. SCREENING TRANSFORMANTS Lab session 6-8
3. EXPRESSION, PURIFICATION, AND ANALYSIS OF RECOMBINANT PROTEINS FROM BACTERIA Lab session 9-12b
4. ANALYSIS OF mRNA LEVELS Lab Session 13-17
5. MODULATION OF GENE EXPRESSION Lab Session 18-30
6. IN SILICO TOOLS Lab Session 31-33
2. SCREENING TRANSFORMANTS Lab session 6-8
3. EXPRESSION, PURIFICATION, AND ANALYSIS OF RECOMBINANT PROTEINS FROM BACTERIA Lab session 9-12b
4. ANALYSIS OF mRNA LEVELS Lab Session 13-17
5. MODULATION OF GENE EXPRESSION Lab Session 18-30
6. IN SILICO TOOLS Lab Session 31-33
ISBN:
9780443267147
Page Count:
528
Retail Price
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Access to teacher/student resources is available to registered users with approved inspection copies or confirmed adoptions. To review this material, please request an inspection copy.
Upper-level undergraduate and intro-level graduate laboratory courses